Specific transcription of orthopox virus DNA by HeLa cell RNA polymerase II

A HeLa cell extract was used to transcribe DNA isolated from cowpox virus. Truncated templates generate accurately initiated run-off transcripts of discrete sizes and whose sensitivity to inhibition by alpha-amanitin indicates synthesis by cell RNA polymerase II. A mapped restriction fragment of wild-type cowpox DNA contains specific sites of initiation which are not detected in the geographically equivalent fragment from a cowpox mutant having a defined sequence rearrangement in this region.