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In Vitro Study of Endotoxin Adsorption by a Polymyxin B-Immobilized Fiber Column

Chizuru Yamashita1, Kazuhiro Moriyama2, Daisuke Hasegawa1

  • 1Department of Anesthesiology and Critical Care Medicine, Fujita Health University School of Medicine, Toyoake, Japan.

Blood Purification
|July 4, 2018
PubMed
Abstract

Insights

Polymyxin B-immobilized fiber (PMX-F) columns demonstrate sustained endotoxin removal for septic shock therapy. These PMX-F columns effectively adsorb endotoxin continuously for at least 24 hours.

Area of Science:

  • Biomedical Engineering
  • Critical Care Medicine
  • Pharmacology

Background:

  • Polymyxin B-immobilized fiber (PMX-F) columns are utilized for septic shock treatment.
  • Previous studies confirmed efficacy for 2-hour applications.
  • Extended endotoxin adsorption capacity of PMX-F columns remains under investigation.

Purpose of the Study:

  • To evaluate the endotoxin adsorption capability of PMX-F columns beyond 2-hour treatment durations.
  • To test the hypothesis that PMX-F columns can remove endotoxin for over 2 hours.

Main Methods:

  • A closed-circuit system was designed using PMX-F columns (PMX-01R) or sham controls.
  • Inactivated fetal bovine serum served as the perfusate.
  • Endotoxin was continuously infused for 24 hours, with perfusate concentrations measured periodically.

Main Results:

  • Endotoxin adsorption by the PMX-01R column exhibited continuous, near-linear increase.
  • The PMX-01R column demonstrated consistent endotoxin adsorption throughout the 24-hour study period.

Conclusions:

  • PMX-F columns maintain endotoxin adsorption capacity for a minimum of 24 hours.
  • Findings suggest PMX-F columns are suitable for extended clinical endotoxin removal in septic shock management.
Keywords:
24-h hemoperfusionAdsorption saturationContinuous endotoxin injectionEndotoxin adsorptionPolymyxin B-immobilized fiber

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