Related Experiment Video
Updated: Feb 8, 2026

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
A Micelle-Enhanced Spectrofluorimetric Determination of Pirfenidone: Application to Content Uniformity Testing and
Naga Gayatri Sambhani1, Valsala Madhavan Nair Biju2
1Department of Chemistry, National Institute of Technology, Tiruchirappalli, Tamil Nadu, 620 015, India.
Abstract:
The present manuscript gives a detailed account of highly selective, validated and sensitive method for quantification of pirfenidone in its pharmaceutical dosage forms and spiked human urine. The developed method is relied on the systematic study of the fluorescence action of Pirfenidone in Tween - 80 micellar medium. The Pirfenidone exhibits strong fluorescence at λem 396 nm upon excitation at λex 318 nm in Tween -80 medium. The fluorescence - concentration plot was linear over concentration range of 0.5 - 5 μg/mL. There was greater extent (1.02 fold) of enhancement in fluorescence intensity in presence of tween - 80 with very low limit of detection and quantitation of 0.04 μg/mL and 0.11 μg/mL respectively. The application of developed methodology is successfully applied to content uniformity testing and spiked human urine. The proposed study was successfully applied for analysis of pirfenidone in commercially available pharmaceutical formulations.
Related Concept Videos
Filtration and Urine Formation
Urine Studies II: Urine Culture and Sensitivity Test
Uniform Distribution
Two essential properties of this distribution are
Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems
Uniform Circular Motion
Non-uniform Circular Motion
For example, such...

