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Related Experiment Videos

Ionophorous activity and murine B lymphocyte mitogens.

D L Rosenstreich, R Blumenthal

    Journal of Immunology (Baltimore, Md. : 1950)
    |January 1, 1977
    PubMed
    Summary

    Most ionophores do not activate B cells, and most B cell activators do not affect ion transport. However, some polymeric proteins like EIM and KLH show both activities, suggesting a novel mechanism for B cell activation.

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    Area of Science:

    • Immunology
    • Biochemistry
    • Cell Biology

    Background:

    • The relationship between ionophorous activity and B cell mitogenesis is not well understood.
    • Most known ionophores lack B cell mitogenic activity, and most B cell mitogens are not ionophorous.

    Purpose of the Study:

    • To investigate the connection between ionophorous properties and B cell mitogen activity.
    • To explore the mechanism by which certain molecules activate B lymphocytes.

    Main Methods:

    • Tested ionophoric and mitogenic activities of various compounds using bilayer lipid membrane (BLM) apparatus and mouse spleen cells.
    • Assessed endotoxin contamination using limulus lysate clotting and rabbit pyrogenicity assays.
    • Investigated the role of polymeric structure and heat denaturation on activity.

    Main Results:

    • Excitability-inducing material (EIM) and keyhole limpet hemocyanin (KLH), both high molecular weight polymeric proteins, exhibited potent B cell mitogenic and channel-forming ionophore activities.
    • Mitogenic activities were independent of endotoxin contamination.
    • Loss of polymeric structure or heat denaturation affected ionophoric activity but not always mitogenicity, indicating ionophoric activity is not essential for B cell activation.

    Conclusions:

    • B cell mitogens do not necessarily function as primary ionophores.
    • Proposed that intercalation of these molecules into the cell membrane lipid bilayer initiates B cell activation.

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