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Updated: Feb 8, 2026
09:49
Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
Published on: July 5, 2019
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A Comprehensive Method for Detecting Fusion Genes in Paediatric Brain Tumours
Akihide Kondo1, Yuzaburo Shimizu2, Satoshi Adachi2
1Department of Neurosurgery, Juntendo University Faculty of Medicine, Tokyo, Japan knd-aki@juntendo.ac.jp.
Cancer Genomics & Proteomics
|July 7, 2018
Summary
A new pyrosequencing method rapidly detects KIAA1549-BRAF fusion genes in pediatric brain tumors. This advancement aids research into fusion genes driving tumorigenesis.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Fusion genes are critical drivers of tumorigenesis.
- Reproducible methods for detecting fusion genes are lacking.
- Paediatric brain tumours are a focus of fusion gene research.
Purpose of the Study:
- To evaluate a comprehensive method for detecting fusion genes in paediatric brain tumours.
- To assess the speed and reproducibility of the detection method.
Main Methods:
- Examined 19 paediatric brain tumours across five types.
- Utilized a pyrosequencing-based method.
- Involved RNA isolation, cDNA synthesis, and real-time PCR.
Main Results:
- Successfully detected KIAA1549-BRAF fusion in 14/19 patients.
- Provided information on fusion breakpoints within 2 hours.
- Identified KIAA1549-BRAF fusion variants quickly.
Conclusions:
- The evaluated method is comprehensive and effective for detecting fusion genes in paediatric brain tumours.
- The method offers rapid identification of KIAA1549-BRAF fusion variants.
- Findings support further research into the role of fusion genes in tumourigenesis.
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