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Updated: Aug 14, 2026

Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells
Published on: July 27, 2018
Characterization of growth parameters of a human hepatoma cell line cultured under serum-free conditions
Abstract:
The PLC/PRF/5 human hepatoma cell line has at least four complete series of hepatitis B virus (HBV)-DNA sequences integrated into the host DNA and produces hepatitis B surface antigen (HBsAg) and several serum proteins in the culture medium. In order to study serum proteins and HBsAg released by these cells under controlled conditions, the serum-free growth of several human hepatoma-derived cell lines was recently investigated. In this paper the growth of PLC/PRF/5 human hepatoma cell line in a serum- and hormone-free medium was investigated. The results represent a tool which might be used in pharmacological research, studies on hormone-binding and virus gene expression.

