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Updated: Feb 8, 2026
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An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
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Intraoperative Ureter Visualization Using a Novel Near-Infrared Fluorescent Dye
Sakkarapalayam M Mahalingam1, Fernando Dip2, Marco Castillo2
1Department of Chemistry and Institute for Drug Discovery , Purdue University , West Lafayette , Indiana 47907 , United States.
Molecular Pharmaceutics
|July 7, 2018
Summary
A novel near-infrared (NIR) fluorescent dye, UreterGlow, enables clear visualization of ureters during surgery. This innovation aids in preventing surgical complications and improving patient outcomes.
Area of Science:
- Surgical innovation
- Medical imaging
- Fluorescent dye development
Background:
- Accidental ureter severance during pelvic surgery leads to complications.
- Existing methods for ureter visualization have limitations.
Purpose of the Study:
- To synthesize and characterize a new near-infrared (NIR) fluorescent dye, UreterGlow.
- To evaluate UreterGlow's efficacy in visualizing ureters during surgery.
Main Methods:
- Systemic intravenous injection of UreterGlow (0.1 mg/kg) in Yorkshire pigs.
- Ureter imaging using an NIR fluorescence camera.
- Spectroscopic characterization of UreterGlow's excitation and emission maxima.
Main Results:
- UreterGlow visualized ureters in pigs under NIR illumination 60 minutes post-injection for over 2 hours.
- Ureter peristalsis was observable shortly after injection.
- Ureters were not visible using standard visible light.
Conclusions:
- UreterGlow facilitates real-time ureter imaging, aiding surgical repair.
- Its distinct spectral properties allow differentiation from tumor-imaging dyes.
- This dye offers a promising solution to prevent ureter-related surgical complications.
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