Related Experiment Video
Updated: Feb 8, 2026
15:06
Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
Published on: January 3, 2016
13.4K
A rapid readout for many single plasmonic nanoparticles using dark-field microscopy and digital color analysis
Manish Sriram1, Bijan P Markhali1, Philip R Nicovich2
1School of Chemistry, The University of New South Wales, Sydney 2052, Australia; Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of New South Wales, Sydney 2052, Australia.
Biosensors & Bioelectronics
|July 9, 2018
Summary
This study presents a rapid, low-cost method for analyzing plasmonic nanoparticles using a digital camera and dark-field microscope. This advancement enables faster, high-throughput single nanoparticle biosensing for point-of-care applications.
Area of Science:
- Nanotechnology and Nanoscience
- Biomedical Engineering
- Analytical Chemistry
Background:
- Plasmonic nanoparticles enhance biosensor sensitivity and dynamic range via single nanoparticle assays.
- Localized surface plasmon resonance (LSPR) analysis enables single-molecule detection limits in biosensing.
- Current LSPR technologies face limitations in point-of-care applications due to complex equipment and slow analysis.
Purpose of the Study:
- To develop an advanced LSPR analysis technique for high-throughput single nanoparticle spectral analysis.
- To overcome the limitations of existing LSPR biosensing methods for point-of-care applications.
- To demonstrate a rapid and cost-effective method for analyzing plasmonic nanoparticles.
Main Methods:
- Utilized a CMOS-equipped digital camera and dark-field microscope for LSPR analysis.
- Developed a technique to analyze the maximum wavelength (λmax) of thousands of gold nanospheres in under one second.
- Eliminated the need for a spectrometer in LSPR analysis, improving throughput.
Main Results:
- Achieved rapid analysis of over several thousand gold nanospheres in less than a second.
- Significantly improved the throughput of single particle spectral analysis.
- Successfully demonstrated the technique for detecting interleukin-6 using a core-satellite binding assay.
Conclusions:
- The developed technique offers a high-throughput, cost-effective approach for LSPR analysis.
- This advancement facilitates the development of point-of-care single nanoparticle biosensors.
- The method holds potential for high-throughput and multiplexed biosensing applications.
Keywords:
Color analysisDark-field microscopyGold nanoparticlesLocalized surface plasmon resonancePlasmonic sensorSingle particle detectionMore Related Videos
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