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Related Concept Videos

Structure and Function of Platelets01:18

Structure and Function of Platelets

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The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
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Formation of the Platelet Plug01:22

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The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
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Aggregates Classification01:29

Aggregates Classification

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Aggregate classification is generally based on its size, petrographic characteristics, weight, and source. Size classification ranges from coarse to fine aggregates, defined by the size of the particles. Coarse aggregates are particles that do not pass through ASTM sieve No. 4, and aggregates that pass through the sieve are fine aggregates.
Petrographic classification groups aggregates based on common mineralogical characteristics. Some of the common mineral groups found in aggregates are...
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Bonding and Strength of Aggregate01:12

Bonding and Strength of Aggregate

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The bond between aggregate particles and the cement matrix is significantly influenced by the shape and surface texture of the aggregates. High-strength concretes benefit from a rougher texture, which leads to stronger bonding due to greater adhesion. Angular aggregates with larger surface areas also enhance this bond. The bonding quality, however, is complex to assess as no universally accepted test exists. Good bonding is indicated when a crushed concrete specimen shows some aggregate...
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Specific Gravity of Aggregate01:19

Specific Gravity of Aggregate

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Aggregates typically contain pores, which can be either permeable or impermeable. Considering the pores in the aggregates, the specific gravity of aggregates is defined in three different forms, namely, bulk or gross specific gravity, apparent specific gravity, and absolute specific gravity.
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Bulk Density of Aggregate01:22

Bulk Density of Aggregate

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Bulk density refers to the mass of aggregate particles that would fill a unit volume. The concept of bulk density originates from the inability to pack aggregate particles in a manner that completely eliminates void spaces. Hence, the term bulk refers to the volume that encompasses both the aggregates and the voids. This measurement is crucial when aggregates are batched by volume and is used to convert quantities by mass to volume.
Most natural mineral aggregates, like sand and gravel,...
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Remote platelet function testing using P-selectin expression in patients with recent cerebral ischaemia on clopidogrel.

Stroke and vascular neurology·2020
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Circulating Microparticles in Patients with Symptomatic Carotid Disease Are Related to Embolic Plaque Activity and Recent Cerebral Ischaemia.

Cerebrovascular diseases extra·2019
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Triple versus guideline antiplatelet therapy to prevent recurrence after acute ischaemic stroke or transient ischaemic attack: the TARDIS RCT.

Health technology assessment (Winchester, England)·2018
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Clinical utility of remote platelet function measurement using P-selectin: assessment of aspirin, clopidogrel, and prasugrel and bleeding disorders.

Platelets·2018
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How does measurement of platelet P-selectin compare with other methods of measuring platelet function as a means of determining the effectiveness of antiplatelet therapy?

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Antiplatelet therapy with aspirin, clopidogrel, and dipyridamole versus clopidogrel alone or aspirin and dipyridamole in patients with acute cerebral ischaemia (TARDIS): a randomised, open-label, phase 3 superiority trial.

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Related Experiment Video

Updated: Feb 8, 2026

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
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Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

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Platelet aggregation measured by single-platelet counting and using PFA-100 devices.

Natalia Dovlatova1,2, Stan Heptinstall1,2

  • 1a Thrombosis and Haemostasis Research Group, Division of Clinical Neuroscience , University of Nottingham , Nottingham, United Kingdom.

Platelets
|July 10, 2018
PubMed
Summary
This summary is machine-generated.

Evaluating platelet function is key for assessing bleeding and clotting risks. This study reviews accessible whole-blood tests, including the Platelet Function Analyzer (PFA-100) and single-platelet counting, for broader clinical use.

Keywords:
PFA-100platelet aggregationplatelet testingsingle-platelet counting

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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation

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Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
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Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

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Last Updated: Feb 8, 2026

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
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Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
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Area of Science:

  • Hematology
  • Clinical Pathology
  • Biomedical Engineering

Background:

  • Platelets are vital for hemostasis and thrombosis.
  • Assessing platelet function, particularly aggregation, aids in evaluating bleeding/thrombotic risks and drug effects.
  • Traditional methods are complex, requiring specialized equipment and personnel.

Purpose of the Study:

  • To summarize accessible methods for assessing platelet function in whole blood.
  • To discuss the utility of the Platelet Function Analyzer (PFA-100) as a point-of-care test.
  • To explore the potential of single-platelet counting for wider application.

Main Methods:

  • Review of the Platelet Function Analyzer (PFA-100) for whole-blood platelet function testing.
  • Discussion of single-platelet counting techniques for assessing platelet aggregation in whole blood.
  • Analysis of the accessibility and physiological relevance of these methods.

Main Results:

  • The PFA-100 is an accessible, first-line point-of-care test for whole-blood platelet function.
  • Single-platelet counting is a promising, albeit less established, whole-blood method for assessing platelet aggregation.
  • Both methods offer more physiologically relevant measurements than traditional in vitro assays.

Conclusions:

  • Accessible whole-blood platelet function tests like the PFA-100 and single-platelet counting can improve risk assessment.
  • Further development of single-platelet counting could lead to wider clinical application.
  • These methods offer a more physiologically relevant and accessible approach to platelet function analysis.