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Updated: Feb 8, 2026

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS
Published on: May 3, 2011
Spectral Imaging Analysis for Ultrasensitive Biomolecular Detection Using Gold-Capped Nanowire Arrays
Yi-Hsin Tai1, Po-Han Fu2, Kuang-Li Lee3
1Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. yhtai@gate.sinica.edu.tw.
Abstract:
A spectral integration combined with a threshold method for the analysis of spectral scanning surface plasmon resonance (SPR) images can significantly increase signal recognition at low concentration of antibody solution. The 12-well SPR sensing plates consisted of gold-capped nanowire arrays with 500-nm period, 80-nm linewidth and 50-nm gold thickness which were used for generating multiple SPR images. A threshold method is introduced to eliminate background noises in spectral scanning images. Combining spectral integration and the threshold method, the detection limit of antibody concentration was 1.23 ng/mL. Using multiple-well SPR sensing plates and the proposed analytical method, multiple kinetic responses with spectral and spatial information on different sensing areas can be sensitively measured.
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