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Related Experiment Videos

MicroRNA-146a expression and microRNA-146a rs2910164 polymorphism in Behcet's disease patients.

W Ibrahim1, B R Sakr2, E Obaya1

  • 1Department of Medical biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.

Clinical Rheumatology
|July 11, 2018
PubMed
Summary

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MicroRNA-146a levels are elevated in Egyptian Behcet's disease (BD) patients, correlating with eye and vascular activity. The GG genotype of microRNA-146a rs2910164 may increase BD susceptibility, while the CC genotype appears protective.

Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Rheumatology

Background:

  • Behcet's disease (BD) is a chronic, multisystem inflammatory disorder of unknown cause.
  • Key features include oral ulcers, genital ulcers, skin lesions, and ocular and joint inflammation.

Purpose of the Study:

  • To investigate microRNA-146a expression and its gene polymorphism (rs2910164) in Egyptian BD patients.
  • To explore the association between microRNA-146a and BD clinical manifestations and disease activity.

Main Methods:

  • A case-control study involving 47 Egyptian BD patients and 50 healthy controls.
  • Serum microRNA-146a levels were quantified using real-time polymerase chain reaction (RT-PCR).
  • MicroRNA-146a rs2910164 single nucleotide polymorphism (SNP) genotyping was performed using RT-PCR.
Keywords:
Behcet’s diseaseGenotypeMicroRNASingle-nuclear polymorphism

Related Experiment Videos

Main Results:

  • Serum microRNA-146a expression was significantly higher in BD patients compared to controls (P < 0.001).
  • Elevated microRNA-146a expression showed a significant association with ocular (P = 0.033) and vascular (P = 0.041) activity in BD.
  • The GG genotype of rs2910164 was more frequent in BD patients (59.6%) than controls (24%), while the CC genotype was less frequent in BD patients (8.5%) compared to controls (12%).

Conclusions:

  • Serum microRNA-146a is significantly upregulated in Egyptian BD patients.
  • MicroRNA-146a expression levels are linked to ocular and vascular activity in Behcet's disease.
  • The rs2910164 GG genotype may confer susceptibility to BD, whereas the CC genotype might offer protection.