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Updated: Feb 8, 2026

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
Published on: April 20, 2018
Isolation and Characterization of Human Alveolar Type II Cells
Beata Kosmider1,2,3, Robert J Mason4, Karim Bahmed5,6
1Department of Thoracic Medicine and Surgery, Temple University, Philadelphia, PA, USA. beata.kosmider@temple.edu.
Abstract:
Alveolar type II (ATII) cells synthesize, store, and secrete pulmonary surfactant and restore the epithelium after damage to the alveolar epithelium. Isolation of human ATII cells provides a valuable tool to study their function under normal and pathophysiological conditions. Moreover, maintenance of their differentiated phenotype in vitro allows further study of their function. Here we describe a protocol for efficient ATII cell isolation, characterization, and culture.
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