Evoking and tracking zebrafish eye movement in multiple larvae with ZebEyeTrack
Florian A Dehmelt1, Adam von Daranyi2, Claire Leyden1,3
1Werner Reichardt Centre for Integrative Neuroscience and Institute of Neurobiology, University of Tübingen, Tübingen, Germany.
Nature Protocols
|July 11, 2018
Summary
This study introduces ZebEyeTrack, a novel software for automated eye movement tracking in larval zebrafish. It enables precise, real-time measurement critical for behavioral vision research and neural circuit studies.
Area of Science:
- Neuroscience
- Behavioral Biology
- Biomedical Engineering
Background:
- Accurate eye movement measurement is vital for behavioral vision research.
- Zebrafish are a key model organism for studying visual neural circuits.
- Existing eye-tracking solutions for larval zebrafish are limited.
Purpose of the Study:
- To present a protocol for automated, real-time measurement of horizontal eye position in larval zebrafish.
- To introduce ZebEyeTrack, a LabVIEW-based software for eye tracking and experimental control.
- To provide customizable source code and a streamlined version (ZebEyeTrack Light).
Main Methods:
- Developed custom LabVIEW-based software (ZebEyeTrack) for automated eye tracking.
- Implemented real-time measurement of angular horizontal eye position in immobilized larval fish.
- Integrated stimulus design, visual stimulation, eye tracking, real-time analysis, closed-loop control, and data recording.
Main Results:
- ZebEyeTrack enables automated, real-time angular horizontal eye position measurement for up to six fish.
- The software synchronizes multiple experimental aspects, including stimulus presentation and closed-loop control.
- Digitized eye positions facilitate analysis of tuning curves, optokinetic gain, and other custom metrics.
- Semi-automated analysis of optokinetic response (OKR) performance is available post-experiment or from existing videos.
Conclusions:
- ZebEyeTrack provides a ready-to-use solution for precise eye movement measurement in larval zebrafish.
- The software supports various applications, including psychophysics, optogenetics, and calcium imaging.
- This tool advances behavioral vision research using zebrafish as a model organism.
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