Related Experiment Video
Updated: Feb 8, 2026

09:20
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
Published on: September 2, 2013
28.4K
A human proteomic dataset from untreated and depleted/enriched serum samples
Salvatore Pisanu1, Grazia Biosa1, Laura Carcangiu1
1Porto Conte Ricerche, Science and Technology Park of Sardinia, Alghero, Sassari, Tramariglio, Italy.
Data in Brief
|July 11, 2018
Summary
This study evaluated seven commercial kits for human serum proteomics. Proteomic datasets from enriched and depleted fractions were analyzed using mass spectrometry and Gene Ontology annotation.
Area of Science:
- Proteomics
- Biochemistry
- Molecular Biology
Background:
- Human serum proteomics is crucial for biomarker discovery.
- Effective enrichment and depletion of high-abundance proteins are essential for deep proteome profiling.
- Various commercial kits exist, necessitating comparative evaluation.
Purpose of the Study:
- To present a proteomic dataset from human serum using seven commercial enrichment/depletion kits.
- To compare the performance of these commercial products in human serum sample preparation.
- To provide data for shotgun proteomics studies.
Main Methods:
- Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) analysis.
- Label-free quantitation utilizing spectral counting.
- Gene Ontology (GO) annotation for functional analysis.
Main Results:
- Generation of a comprehensive proteomic dataset from serum fractions.
- Analysis of protein relative abundances and functional annotations.
- Comparative data on the efficacy of seven commercial serum processing kits.
Conclusions:
- The dataset provides valuable insights into human serum proteome composition.
- The comparative analysis aids researchers in selecting appropriate serum enrichment/depletion tools.
- This work supports advancements in biomarker discovery and diagnostic research.
Related Concept Videos
Proteomics
9.8K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.8K
MOSFET: Depletion Mode
867
Depletion-mode MOSFETs represent a unique subset of MOSFET technology, functioning fundamentally differently from their enhancement-mode counterparts. Unlike enhancement MOSFETs, which require a positive gate-source voltage (Vgs) to turn on, depletion-mode MOSFETs are inherently conductive and "normally on" devices.
The primary characteristic of depletion-mode MOSFETs is their ability to conduct current between the drain and source terminals without gate bias. This inherent conductivity...
The primary characteristic of depletion-mode MOSFETs is their ability to conduct current between the drain and source terminals without gate bias. This inherent conductivity...
867
Serum Laboratory Studies, Stool Test, Breath Test
887
Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...
887
Serum Studies: Renal Function Tests
448
Renal function tests are crucial for assessing kidney health, monitoring disease progression, and evaluating the kidneys' efficiency in waste elimination, fluid balance, and electrolyte regulation. These tests offer critical insights into kidney function, even though routine measurements may appear normal until there is a significant decline in the glomerular filtration rate or GFR. Typically, signs of kidney impairment only become evident when the GFR falls to about 50% of its normal level.
448
Sampling Methods: Sample Types
3.3K
Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
3.3K
Sampling Plans
994
Sampling is a crucial step in analytical chemistry, allowing researchers to collect representative data from a large population. Common sampling methods include random, judgmental, systematic, stratified, and cluster sampling.
Random sampling is a method where each member of the population has an equal chance of being selected for the sample. It involves selecting individuals randomly, often using random number generators or lottery-type methods. For example, when analyzing the properties of a...
Random sampling is a method where each member of the population has an equal chance of being selected for the sample. It involves selecting individuals randomly, often using random number generators or lottery-type methods. For example, when analyzing the properties of a...
994

