FRET-based Solid-state Luminescent Glyphosate Sensor Using Calixarene-grafted Ruthenium(II)bipyridine Doped Silica...

Area of Science:

• Nanotechnology
• Environmental Science
• Analytical Chemistry

Background:

• Organophosphorus pesticides like glyphosate pose environmental and health risks.
• Sensitive and selective detection methods are crucial for monitoring glyphosate levels.
• Existing detection methods may lack the required sensitivity or selectivity.

Purpose of the Study:

• To develop a novel FRET-based nanosensor for the selective and sensitive detection of glyphosate.
• To fabricate calixarene-functionalized luminescent nanoparticles for enhanced glyphosate sensing.
• To evaluate the performance of the developed nanosensor in terms of sensitivity, selectivity, and binding affinity.

Main Methods:

• Fabrication of luminescent nanoparticles by grafting p-tert-butylcalix[4]arene onto [Ru(bpy)3]2+ incorporated SiNps.
• Utilizing Förster Resonance Energy Transfer (FRET) as the detection mechanism.
• Characterization of the nanosensor's response to glyphosate using fluorescence spectroscopy and binding isotherm analysis.

Main Results:

• The developed nanosensor exhibited efficient FRET in the presence of glyphosate.
• FRET efficiency increased with increasing glyphosate concentration, showing a gradual response.
• A high binding affinity (1.16×10^7 M^-1) and a low limit of detection (7.91×10^-7 M) were achieved.
• The nanosensor demonstrated effective performance in real sample analysis.

Conclusions:

• Calixarene-functionalized luminescent nanoparticles provide a sensitive and selective platform for glyphosate detection.
• The FRET-based mechanism enables robust detection with a low limit of detection, surpassing EPA recommendations.
• The developed nanosensor shows promise for practical environmental monitoring applications.