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A "Hole Punched Plate" method for easy generation and harvesting of microconidia in the dermatophyte Trichophyton
1Santa Fe BioLabs LLC, Fort Worth, TX, USA.
Heliyon
|July 12, 2018
Abstract:
Handling of the medically important dermatophyte Trichophyton rubrum in the laboratory typically requires the generation of spores - for storage, treatment and plating when needed. The described method allows technically simple but efficient generation and harvesting of microconidia by cutting holes in Sabouraud dextrose agar medium that is covered by a mature T. rubrum mycelium.
Insights
A simple method efficiently generates Trichophyton rubrum microconidia spores for laboratory use. This technique involves cutting holes in agar to harvest spores from the mycelium.
Area of Science:
- Medical Mycology
- Microbiology
Background:
- Trichophyton rubrum is a medically significant dermatophyte.
- Laboratory handling of T. rubrum often necessitates spore generation for various applications.
Purpose of the Study:
- To describe a simple and efficient method for generating and harvesting microconidia from T. rubrum.
Main Methods:
- The method involves culturing T. rubrum on Sabouraud dextrose agar.
- Holes are cut into the agar medium overlaid with mature T. rubrum mycelium.
- This facilitates the generation and subsequent harvesting of microconidia.
Main Results:
- The described technique provides a technically simple yet efficient way to produce T. rubrum microconidia.
- The method allows for straightforward harvesting of the generated spores.
Conclusions:
- This approach offers a practical solution for routine laboratory spore generation of T. rubrum.
- The ease of implementation makes it suitable for various research and diagnostic purposes.