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Updated: Feb 8, 2026

Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
Published on: March 27, 2014
Bioenergetics of life, disease and death phenomena
Andrzej Kasperski1, Renata Kasperska2
1Department of Biotechnology, Faculty of Biological Sciences, University of Zielona Gora, ul. Szafrana 1, 65-516, Zielona Gora, Poland. A.Kasperski@wnb.uz.zgora.pl.
Abstract:
In this article, some new aspects of unified cell bioenergetics are presented. From the perspective of unified cell bioenergetics certain subsequent stages of cancer development, from initiation stage, through transformation to metastasis, are analyzed. Here we show that after transformation, cancer cells are permanently exposed to reactive oxygen species, that causes continual random DNA mutations and as a result genome and chromosomal destabilizations. The modern cancer attractor hypothesis has been extended in explaining cancer development. Discussion is conducted in light of current cancerogenesis research, including bioenergetic cancer initiation, the somatic mutation theory and the tissue organization field theory. In the article reasons complicating the discovery of patterns of cancer genome changes and cancer evolution are presented. In addition certain cancer therapeutic aspects are given attention to.
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