Related Experiment Video
Updated: Feb 7, 2026

Biofilm Removal Using Carbon Dioxide Aerosols without Nitrogen Purge
Published on: November 6, 2016
[Nitrogen Removal of Double-bacteria-layer System via PN/A Process]
Jing-Yi Xu1, Jun Du1, Yi-Feng Yang1
1College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
A novel PN/A double-bacteria-layer system efficiently removes ammonia nitrogen (NH4+-N) using immobilized nitrification and anaerobic ammonia oxidation (ANAMMOX) sludge. This system enhances stability and nitrogen removal, even with limited dissolved oxygen (DO).
Area of Science:
- Environmental Science
- Microbiology
- Chemical Engineering
Background:
- Wastewater treatment often faces challenges with efficient nitrogen removal.
- Simultaneous nitrification and anaerobic ammonia oxidation (SNA) processes require optimized microbial consortia and reactor configurations.
- Microbial immobilization is a key technology for enhancing the performance of wastewater treatment systems.
Purpose of the Study:
- To develop and evaluate a novel PN/A double-bacteria-layer system for enhanced nitrogen removal.
- To investigate the roles of ammonia-oxidizing bacteria (AOB) and ANAMMOX bacteria in the system.
- To assess the system's performance under varying influent conditions and limited dissolved oxygen (DO).
Main Methods:
- Acclimation of nitrification and ANAMMOX sludge using Sequencing Batch Reactor (SBR) and Membrane Bioreactor (MBR) respectively.
- Construction of a PN/A double-bacteria-layer system with microbial immobilization.
- Short-term and long-term experiments to evaluate nitrogen removal efficiency, stability, and DO utilization.
Main Results:
- The PN/A system achieved 98.8% NH4+-N removal in short-term tests, with distinct roles for AOB and ANAMMOX bacteria.
- Stable nitrogen removal efficiency of ~80% was maintained at low DO levels (1.0 mg·L-1) with influent NH4+-N concentrations of 200-400 mg·L-1.
- Compared to the control, the PN/A system showed significantly higher nitrogen removal efficiency under limited DO conditions.
Conclusions:
- The PN/A double-bacteria-layer system effectively integrates nitrification and ANAMMOX processes for superior nitrogen removal.
- Microbial immobilization and the double-bacteria-layer design enhance DO utilization and system stability.
- This innovative system offers a promising solution for efficient and stable removal of ammonia nitrogen in wastewater treatment.
Related Concept Videos
The Nitrogen Cycle
Coordination of Gene Expression Processes in Bacteria
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
Inorganic Nitrogen Assimilation
Chirality at Nitrogen, Phosphorus, and Sulfur
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...
The Roles of Bacteria and Fungi in Plant Nutrition

