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Tumour necrosis factor-related apoptosis-inducing ligand expression in patients with diabetic nephropathy

Wei-Wei Chang1, Wei Liang2, Xin-Ming Yao3

  • 11 Department of Epidemiology and Biostatistics, School of Public Health, Wannan Medical College, China.

Journal of the Renin-Angiotensin-Aldosterone System : JRAAS
|July 13, 2018
PubMed
Abstract

Insights

Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) levels were significantly lower in patients with diabetes mellitus and diabetic nephropathy. This finding suggests TRAIL may play a role in diabetic nephropathy pathogenesis.

Area of Science:

  • Nephrology
  • Immunology
  • Endocrinology

Background:

  • Diabetic nephropathy (DN) is a major complication of diabetes mellitus.
  • Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) is involved in immune responses and apoptosis.
  • The role of TRAIL in DN pathogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the expression profile of TRAIL in patients with DN.
  • To compare TRAIL levels in patients with diabetes mellitus (DM), DN, and healthy controls.

Main Methods:

  • Real-time polymerase chain reaction (PCR) to quantify TRAIL mRNA in peripheral blood mononuclear cells (PBMCs).
  • Enzyme-linked immunosorbent assay (ELISA) to measure serum soluble TRAIL (sTRAIL) and cytokine levels.
  • Study included 126 Chinese subjects: 42 DM patients, 42 DN patients, and 42 healthy controls.

Main Results:

  • TRAIL mRNA and sTRAIL levels were significantly decreased in DM and DN patients compared to controls (P < 0.05).
  • DN patients exhibited elevated levels of IL-1, IL-6, TNF-α, and MCP-1 compared to controls.
  • Serum TRAIL levels positively correlated with TRAIL mRNA levels across all subjects (P < 0.05).

Conclusions:

  • Reduced TRAIL expression is associated with DM and DN.
  • Elevated pro-inflammatory cytokines are present in DN patients.
  • These findings support further investigation into TRAIL's role in DN pathogenesis.
Keywords:
Diabetic nephropathyIL-1IL-6TNF-αTRAIL

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