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Related Experiment Video

Updated: Feb 7, 2026

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Injectable Enzymatically Cross-linked Hydrogels with Light-Controlled Degradation Profile.

Yilong Cheng1,2, Chaoliang He1, Kaixuan Ren1

  • 1Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, 130022, China.

Macromolecular Rapid Communications
|July 13, 2018
PubMed
Summary

Researchers developed advanced hydrogels from modified 4-arm poly(ethylene glycol) (PEG). These injectable hydrogels offer tunable properties and light-controlled degradation, showing potential for biomedical applications.

Keywords:
enzymatic cross-linkinginjectable hydrogelsphoto-degradationpoly(ethylene glycol)

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Tissue Engineering

Background:

  • Hydrogels are crucial in biomedical applications due to their structural similarity to the extracellular matrix.
  • Developing hydrogels with controlled formation, degradation, and injectability remains a significant challenge.
  • Existing hydrogels often lack precise control over degradation kinetics and mechanical properties.

Purpose of the Study:

  • To synthesize and characterize advanced hydrogels with facile formation and injectability.
  • To achieve light-controlled degradation of hydrogels via a photo-cleavage mechanism.
  • To investigate the tunability of hydrogel properties (gelation time, mechanical strength, porous structure) and their in vitro and in vivo performance.

Main Methods:

  • Modification of 4-arm poly(ethylene glycol) (PEG) with 2-nitrobenzyl (NB) and phenol groups.
  • Enzymatic cross-linking of modified PEG precursors using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2).
  • Characterization of hydrogel properties including gelation kinetics, mechanical strength, and porous structure.
  • Assessment of UV light-induced degradation via photo-cleavage of NB ester bonds.
  • In vitro cytotoxicity evaluation using mouse fibroblast L929 cells.
  • In vivo injectability and manipulation assessment.

Main Results:

  • Facile formation of enzymatically cross-linked hydrogels from modified 4-arm PEG precursors.
  • Tunable gelation time, mechanical strength, and porous structure controlled by HRP and H2O2 concentrations.
  • Controlled degradation of hydrogels under UV light irradiation through photo-cleavage of the NB ester bond.
  • Negligible cytotoxicity observed in vitro toward mouse fibroblast L929 cells.
  • Successful manipulation and injectability demonstrated in vivo.

Conclusions:

  • The developed hydrogels offer a promising platform for advanced biomedical applications requiring tunable properties and controlled degradation.
  • The facile enzymatic cross-linking and light-triggered degradation provide precise control over hydrogel behavior.
  • The biocompatibility and injectability of these hydrogels support their potential use in minimally invasive therapies and tissue engineering.