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Updated: Feb 7, 2026

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Published on: June 5, 2015
Expression of New Recombinant IgG-Binding Polypeptides and Analysis of Their Capacity to Bind Human IgG
1Department of Molecular Microbiology, Institute of Experimental Medicine, St. Petersburg, Russia.
Abstract:
Protein G is present in group G streptococcus strain (G4223); the IgG-binding part of this protein contains three IgG-binding domains and binds human IgG with very high activity. We obtained two recombinant polypeptides G4223 and G14223 with high IgG-binding activity. Polypeptide G14223 consisting of three IgG-binding domains and W region has higher molecular weight and is characterized by higher affinity for IgG than polypeptide G4223 consisting of only three IgG-binding domains. It was shown that polypeptide affinity depends on its structure and size.
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