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Updated: Feb 7, 2026

Preparation of High-Temperature Sample Grids for Cryo-EM
Published on: July 26, 2021
Cryo-EM structure of the polycystin 2-l1 ion channel
Raymond E Hulse1,2, Zongli Li3, Rick K Huang1
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
Abstract:
We report the near atomic resolution (3.3 Å) of the human polycystic kidney disease 2-like 1 (polycystin 2-l1) ion channel. Encoded by PKD2L1, polycystin 2-l1 is a calcium and monovalent cation-permeant ion channel in primary cilia and plasma membranes. The related primary cilium-specific polycystin-2 protein, encoded by PKD2, shares a high degree of sequence similarity, yet has distinct permeability characteristics. Here we show that these differences are reflected in the architecture of polycystin 2-l1.
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