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Electron Microscopy for the Analysis of Peripheral Nerve Myelin
Cinzia Ferri1, Angelo Quattrini2, Maurizio D'Antonio3
1Division of Genetics and Cell Biology, San Raffaele Scientific Institute, Milan, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|July 15, 2018
Summary
This study details a protocol for preparing mouse peripheral nerve sections for transmission electron microscopy (TEM). This method allows for high-resolution visualization of peripheral myelin structure and pathology.
Area of Science:
- Neuroscience
- Cell Biology
- Microscopy
Background:
- Transmission electron microscopy (TEM) offers high-resolution imaging of cellular ultrastructure.
- Effective sample preparation is crucial for successful TEM analysis.
- Understanding peripheral nerve and myelin structure is vital in neuroscience.
Purpose of the Study:
- To present a detailed protocol for preparing semithin and ultrathin sections of mouse peripheral nerves.
- To enable high-resolution visualization of peripheral myelin using TEM.
- To facilitate the study of structural and pathological features of peripheral myelin.
Main Methods:
- Protocol development for mouse peripheral nerve sample preparation.
- Sectioning into semithin and ultrathin slices.
- Transmission electron microscopy for ultrastructural analysis.
Main Results:
- Successful preparation of mouse peripheral nerve sections for TEM.
- High-resolution images of peripheral myelin structure were obtained.
- Pathological features of peripheral myelin were visualized.
Conclusions:
- The described protocol is effective for TEM analysis of mouse peripheral nerves.
- This method aids in the detailed study of peripheral myelin.
- The protocol supports research into peripheral nerve diseases and conditions.
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