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Related Experiment Video

Updated: Feb 7, 2026

Lipidomics and Transcriptomics in Neurological Diseases

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

Lipidomics Profiling of Myelin.

Chunyan Wang¹, Juan Pablo Palavicini¹, Xianlin Han²

¹Barshop Institute for Longevity and Aging Studies, University of Texas Health San Antonio, San Antonio, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 15, 2018

Summary

This study details multidimensional mass spectrometry shotgun lipidomics (MDMS-SL) for analyzing brain lipidomes, focusing on myelin lipids. The method offers comprehensive lipid quantification for neuroscience research.

Keywords:

Brain Lipid metabolism Mass spectrometry Myelin Shotgun lipidomics

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Shotgun Lipidomics of Rodent Tissues

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Area of Science:

Neuroscience
Lipidomics
Biochemistry

Background:

Lipidomics enables quantitative analysis of numerous lipid species in biological samples.
Recent research underscores the significance of lipidomics in understanding myelin biology.
Brain lipid metabolism and myelin composition are complex and crucial for neurological function.

Purpose of the Study:

To provide a detailed protocol for applying multidimensional mass spectrometry shotgun lipidomics (MDMS-SL) in neuroscience research.
To emphasize the analysis of brain lipidomes, with a specific focus on myelin lipids.
To outline the complete workflow from sample preparation to bioinformatics analysis.

Main Methods:

Detailed description of sample preparation: brain tissue harvesting, homogenization, and lipid extraction.
Application of MDMS-SL for unbiased, wide-coverage determination and quantification of lipid content.
Adaptability of the method for whole brain tissue or specific regions like the hippocampus and cerebellum.

Main Results:

The MDMS-SL approach allows for comprehensive lipid profiling of brain samples.
Quantification of hundreds of lipid species with high accuracy and coverage.
Successful application to various brain regions, demonstrating method's versatility.

Conclusions:

MDMS-SL is a robust method for detailed brain lipidome analysis in neuroscience.
This approach is expected to significantly advance the understanding of myelin biology.
The protocol facilitates new insights into lipid metabolism within the central nervous system.