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Related Experiment Video

Updated: Nov 10, 2025

Shotgun Lipidomics of Rodent Tissues

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Mass Spectrometry-Based Shotgun Lipidomics for Cancer Research.

Jianing Wang¹, Chunyan Wang¹, Xianlin Han^2,3

¹Barshop Institute for Longevity and Aging Studies, San Antonio, TX, USA.

Advances in Experimental Medicine and Biology

|April 1, 2021

Summary

This summary is machine-generated.

Shotgun lipidomics offers high-throughput, accurate analysis of cellular lipids using mass spectrometry. This method is increasingly vital for advancing quantitative biology and metabolomics in cancer research.

Keywords:

Cancer lipidomics Direct infusion-based shotgun lipidomics Imaging lipidomics Mass spectrometry Shotgun lipidomics

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Area of Science:

Biochemistry
Analytical Chemistry
Cancer Biology

Background:

Lipidomics is crucial for understanding cellular functions and disease mechanisms.
Cancer research increasingly requires quantitative biological and metabolomic data.
Shotgun lipidomics provides a powerful tool for systematic lipid analysis.

Purpose of the Study:

To overview the principles and approaches of shotgun lipidomics.
To highlight the applications of shotgun lipidomics in cancer research.
To emphasize the growing demand for quantitative lipidomics.

Main Methods:

Direct analysis of lipid extracts from biological samples.
Utilizes mass spectrometry for lipid identification and quantification.
Employs a high-throughput, systematic approach.

Main Results:

Shotgun lipidomics enables large-scale analysis of lipid composition, structure, and quantity.
The approach offers high throughput and quantitative accuracy, including absolute quantification.
Demonstrates utility in various cancer research contexts.

Conclusions:

Shotgun lipidomics is a valuable technique for in-depth cancer research.
Its quantitative accuracy and high throughput meet the demands of modern metabolomics.
Further applications in cancer biology are anticipated.