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Related Experiment Video

Updated: Feb 7, 2026

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

Published on: August 15, 2011

A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality.

Tanya L Daigle¹, Linda Madisen¹, Travis A Hage¹

¹Allen Institute for Brain Science, Seattle, WA 98109, USA.

|July 16, 2018

Summary

Researchers developed new transgenic mouse lines for precise brain cell study. The TIGRE2.0 platform offers advanced tools for genetic targeting, enabling better identification and manipulation of neuronal populations.

Keywords:

Cre Flp TIGRE calcium sensor cell type channelrhodopsin optogenetics reporter transgenic mice voltage sensor

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Last Updated: Feb 7, 2026

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

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AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

Area of Science:

Neuroscience
Genetics
Molecular Biology

Background:

Genetic tools are crucial for understanding brain cell function.
Existing transgenic mouse lines have limitations in specificity and expression levels.

Purpose of the Study:

To report a comprehensive set of new driver and reporter transgenic mouse lines.
To introduce the TIGRE2.0 transgenic platform for enhanced genetic targeting in the mouse brain.

Main Methods:

Development of 23 new driver lines for cortical and subcortical cell populations.
Creation of 26 new reporter lines with various molecular tools, including Cre-dependent reporters.
Utilizing the TIGRE2.0 platform for single-copy targeted-insertion transgenesis.

Main Results:

The TIGRE2.0 platform enables high transgene expression levels in diverse neuronal types.
New lines facilitate optical physiology, optogenetics, and sparse labeling of specific cell populations.
Simplified breeding strategies compared to previous TIGRE lines.

Conclusions:

The novel transgenic lines significantly expand the toolkit for high-precision genetic manipulation of mouse brain cells.
These tools will accelerate research in identifying, monitoring, and controlling distinct neuronal populations.
The TIGRE2.0 platform represents a significant advancement in genetic engineering for neuroscience research.