Rapid creation system of morphologically and functionally communicative three-dimensional cell-dense tissue by...

Area of Science:

• Biotechnology
• Tissue Engineering
• Regenerative Medicine

Background:

• Fabricating functional 3D cell-dense tissues typically requires scaffolds and extended incubation times.
• Achieving rapid, robust cell-cell and cell-surface adhesion is crucial for tissue development.
• Existing methods often result in fragile tissues or prolonged fabrication processes.

Purpose of the Study:

• To develop a rapid fabrication system for scaffold-free, 3D cell-dense tissues using centrifugation.
• To investigate the effect of centrifugation on cell adhesion and tissue formation.
• To demonstrate the system's applicability for creating both muscle and cardiac tissues.

Main Methods:

• Utilized centrifugation (80 x g, 37 °C, 30 min) to enhance cell adhesion to culture surfaces.
• Employed temperature-responsive culture surfaces for efficient tissue harvesting.
• Cultivated C2C12 myoblasts and human induced pluripotent stem (iPS) cell-derived cardiomyocytes.

Main Results:

• Achieved rapid fabrication of 200 μm-thick 3D myoblast tissues in 1.5 hours, compared to fragile tissues in 7.5 hours without centrifugation.
• Demonstrated significantly accelerated cell adhesion without notable cell damage.
• Successfully created electrically/functionally communicative, thicker human iPS cell-derived cardiac tissues.

Conclusions:

• A centrifugation-based system significantly shortens the creation time of 3D cell-dense tissues.
• This method promotes robust cell adhesion and tissue integrity, overcoming limitations of traditional approaches.
• The developed system holds promise for advancing tissue engineering and regenerative medicine applications.