Toxicity of imidazoles ionic liquid [C₁₆mim]Cl to Hela cells

Our study aimed to evaluate the toxicity of 1-hexadecyl-3-methylimidazolium chloride ([C₁₆min]Cl) on the human cervical carcinoma (Hela) cells. We evaluated toxicity, cell viability, genotoxicity, oxidative stress, apoptosis, and apoptosis-related gene expression in Hela cells following exposure to [C₁₆min]Cl. The results indicated that [C₁₆min]Cl inhibited the growth of Hela cells, decreased cell viability, induced DNA damage and apoptosis, inhibited superoxide dismutase, decreased glutathione content, as well as increased the cellular malondialdehyde level of Hela cells. Moreover, [C₁₆min]Cl induced changes in the transcription of p53, Bax and Bcl-2, suggesting that the p53 and Bcl-2 family might have been involved in the cytotoxicity and apoptosis induced by [C₁₆min]Cl in Hela cells. Taken together, these results revealed that [C₁₆min]Cl imparts oxidative stress, genotoxicity, and induces apoptosis in Hela cells; hence, it is not a green solvent.