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Antimicrobial Activity of Antimicrobial Peptide LPcin-YK3 Derived from Bovine Lactophoricin

Ji-Sun Kim1, Ji-Ho Jeong1, Jang-Hee Cho2

  • 1Department of Chemistry, Hankuk University of Foreign Studies, Yong-In 17035, Republic of Korea.

Journal of Microbiology and Biotechnology
|July 20, 2018
PubMed

Insights

A new bovine milk-derived peptide, LPcin-YK3, shows strong antimicrobial activity against Staphylococcus aureus. This safe and effective peptide may offer a solution to antibiotic resistance, particularly for the cosmetics industry.

Area of Science:

  • Biochemistry
  • Microbiology
  • Peptide Science

Background:

  • Lactophoricin (LPcin) is a bovine milk-derived antimicrobial peptide with broad-spectrum activity.
  • Antimicrobial resistance necessitates the development of novel therapeutic agents.

Purpose of the Study:

  • To design and evaluate a shorter analog of LPcin, named LPcin-YK3.
  • To assess the antimicrobial efficacy and safety profile of LPcin-YK3.

Main Methods:

  • LPcin-YK3, a 15-amino acid peptide, was synthesized and its antimicrobial activity determined via Minimum Inhibitory Concentration (MIC) assays.
  • Hemolytic activity assays were performed using mouse and human erythrocytes.
  • Circular dichroism spectroscopy was employed to analyze peptide structure in the presence of lipids.

Main Results:

  • LPcin-YK3 demonstrated potent antimicrobial activity against Staphylococcus aureus with an MIC of 0.62 μg/ml.
  • The peptide exhibited no toxicity to erythrocytes at concentrations up to 40 μg/ml.
  • Circular dichroism confirmed the formation of α-helical structures in the presence of lipids, correlating with antimicrobial action.

Conclusions:

  • LPcin-YK3 is a potent and safe antimicrobial peptide with potential therapeutic applications.
  • This peptide represents a promising new class of agents to combat antibiotic resistance.
  • LPcin-YK3 could be utilized as a therapeutic or natural additive, especially in the cosmetics sector.
Keywords:
Cationic α-helical antimicrobial peptideantimicrobial activityhemolysisnatural preservativestherapeutic additives

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