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Published on: November 29, 2019
Quantitative Photodeprotection Assessment of Caged Resveratrol by Fluorescence Measurement
Shin Hikage1, Yasuhiro Nishiyama1, Yasuo Sasaki1
1Graduate School of Materials and Science, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama-cho, Ikoma, Nara 630-0101, Japan.
Abstract:
For monitoring the regenerated bioactivity of a masked bioactive compound, resveratrol (a luciferase inhibitor) was selected to target such a compound. Caged resveratrol, masked by thiochromone-type photolabile-protecting groups was synthesized in the study. Each caged resveratrol showed lower bioactivity when compared to that shown by the original molecule. After photoirradiation, the original bioactivity was found to be regenerated. Furthermore, the fluorescent compound derived from the thiochromone-type photolabile-protecting groups was generated simultaneously. A linear correlation was observed between the regenerated bioactivity and generated fluorescence intensity. Thus, we quantitatively monitored the recovered bioactivity successfully by measuring the fluorescence.
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