Surface-Selective Control of Cell Orientation on Cyanobacterial Liquid Crystalline Gels

Liquid crystalline hydrogels (LCGs) with layer structures and oriented pores were created using sacran which is a cyanobacterial heteropolysaccharide possessing functional sulfate, carboxylate, and amide groups in common with glycosaminoglycan. The LCG biocompatibility with L929 mouse fibroblasts was confirmed under the appropriate conditions. Enhanced growth and proliferation of L929 cells without exhibiting any toxicity were confirmed. The water contact angle and protein adsorption ability on the LCG were well-controlled by the cross-linking degree. Additionally, fibroblasts were finely oriented on the LCG side face where layer edges made a striped morphology on its surface, whereas the flat top faces of the LCG did not induce any specific cell orientation.