Related Experiment Video
Updated: Feb 7, 2026
Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice
Published on: July 29, 2022
Therapeutic management of peritoneal ascitic sarcomatosis by Ruta graveolens: A study in experimental mice
Sujata Law1, Shalini Sanyal1, Ritam Chatterjee1
1Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108, C.R. Avenue, Kolkata, 700073, West-Bengal, India.
Relevance:
Malignant peritoneal sarcomatosis related ascitic formation often leads to grave consequences but the therapeutic management of the fatal pathophysiological condition remains a rarely discussed issue. The present study investigates the anti-neoplastic activity of the plant alkaloid from Ruta graveolens on ascitic Sarcoma-180 bearing mice as a model of human malignant peritoneal ascites.
Materials And Methods:
The efficacy of the loco-regional administration of Ruta graveolens on tumour cells was explored with cytopathological and cytotoxicological studies, along with the expressional modulation vital regulatory molecules viz. Chk2, c-Myc, CD95 and Aurora kinase.
Results:
The study revealed a series of anti-neoplastic events exerted by Ruta graveolens that included the boosting of anti-tumour immunity, generation of tumour cell cytotoxicity and disruption of cellular energetics which lead to the induction of apoptosis and simultaneous impairment of cell division in tumour cells. Expressional decline of c-Myc oncoproteins and mitosis promoter Aurora kinase A together with up regulation of vital tumour suppressor Chk-2 and apoptosis inducer CD 95 in ascitic tumour cells was also found to be associated with Ruta administration.
Conclusion:
Our observations revealed that loco-regional Ruta administration resulted in the anti-neoplastic effect on peritoneal sarcoma related ascites and the alteration of vital regulatory molecules which depicted the therapeutic utility of Ruta in the management of peritoneal malignant ascites.
Insights
Ruta graveolens demonstrates anti-cancer effects against malignant peritoneal ascites in mice. This plant alkaloid boosts immunity, induces apoptosis, and inhibits tumor cell division, offering therapeutic potential.
Area of Science:
- Pharmacology
- Oncology
- Natural Products
Background:
- Malignant peritoneal sarcomatosis with ascites presents severe complications.
- Effective therapeutic strategies for this condition are limited and underexplored.
Purpose of the Study:
- To investigate the anti-neoplastic activity of Ruta graveolens alkaloid.
- To evaluate its efficacy in a mouse model of human malignant peritoneal ascites.
Main Methods:
- Loco-regional administration of Ruta graveolens in Sarcoma-180 bearing mice.
- Cytopathological and cytotoxicological analyses.
- Assessment of regulatory molecules: Chk2, c-Myc, CD95, Aurora kinase.
Main Results:
- Ruta graveolens enhanced anti-tumor immunity and induced tumor cell cytotoxicity.
- Disrupted cellular energetics, leading to apoptosis and impaired cell division.
- Modulated key regulatory molecules: decreased c-Myc and Aurora kinase A, increased Chk-2 and CD95.
Conclusions:
- Loco-regional Ruta graveolens administration showed significant anti-neoplastic effects on peritoneal sarcoma ascites.
- Alterations in regulatory molecules confirm its therapeutic utility in managing malignant ascites.
Related Concept Videos
01:25Peritoneal Dialysis III: Nursing Management
01:29Appendicitis-II: Diagnostic Studies and Management
Diagnosing Appendicitis
It requires a multifaceted approach, starting with a detailed physical examination to pinpoint the location and nature of the pain and identify any associated symptoms. Laboratory tests play a crucial role. A complete Blood Count (CBC) typically reveals leukocytosis (an increased number of...
01:28Acute Pyelonephritis II: Diagnostic Studies and Management
01:25Peritoneal Dialysis II: Peritoneal Dialysis Systems and Complications
01:13Therapeutic Index
01:30Peritoneal Dialysis I: Introduction and Procedure