Related Experiment Video
Updated: Feb 7, 2026

The Three-Chamber Choice Behavioral Task using Zebrafish as a Model System
Published on: April 14, 2021
Auriculasin from Flemingia philippinensis roots shows good therapeutic indexes on hyperactive behavior in zebrafish
Tuoyi Wang1, Yang Liu2, Huazhen Liu3
1School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou, 215003, China; College of Food and Biological Engineering, Qiqihar University, Qiqihar, 161006, China.
Abstract:
Previously, period1b-/- zebrafish mutants were used to establish an attention deficit hyperactivity disorder (ADHD) model, in which hyperactive behavior was found to be a typical characteristic of ADHD due to down-regulated dopamine levels. Here, we used five prenylated isoflavones from Flemingia philippinensis roots to study their therapeutic effects on hyperactivity behavior in period1b-/- zebrafish. Results of locomotor activity assay showed that auriculasin, one of the prenylated isoflavones, significantly reduced the hyperactivity behavior in period1b-/- zebrafish. Hormone measurement results showed that auriculasin increased melatonin and dopamine content. Results of quantitative real-time polymerase chain reaction showed that auriculasin down-regulated the expression of mao but up-regulated the expression of th and per1b. Thus, auriculasin demonstrated a potential biological effect on dopamine activity to inhibit hyperactivity behavior in the ADHD zebrafish model by regulating circadian clock gene per1b.
Related Concept Videos
Attention-Deficit/Hyperactivity Disorder
Diagnostic Criteria and Symptoms
To diagnose ADHD, symptoms must manifest before age 12 and be evident across multiple settings....
Primary and Secondary Growth in Roots and Shoots
What is Behavior?
Therapeutic Index
Root Mean Square
For example, consider the velocity of gas molecules in a container. The gas molecules are moving in different directions, which might impart positive and negative...
Therapeutic Communication
Verbal communication depends on language or a prescribed way of using words so that people can share information effectively. The critical aspects of verbal...

