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High Resolution Assessment of Spatio-Temporal Changes in O2 Concentration in Root-Pathogen Interaction
Mirco Rodeghiero1, Simonetta Rubol2, Alberto Bellin3,4
1Sustainable Agro-Ecosystems and Bioresources Department, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.
Frontiers in Microbiology
|July 21, 2018
Summary
Fusarium wilt fungus (Fol) rapidly increases metabolic activity upon contact with tomato roots. This study reveals the fungus actively grows towards roots, unlike its random movement in their absence.
Area of Science:
- Plant Pathology
- Mycology
- Plant-Microbe Interactions
Background:
- Fusarium wilt, caused by *Fusarium oxysporum* f. sp. *lycopersici* (Fol), is a major destructive soil-borne disease affecting tomatoes.
- Early-stage metabolic activity and spatial dynamics of the *Fol*-tomato root interaction remain poorly understood.
Purpose of the Study:
- To investigate the metabolic activity and growth patterns of *Fol* in response to tomato roots during early colonization stages.
- To explore the potential of planar optodes and spatial analysis in studying root-pathogen interactions.
Main Methods:
- Combined planar optodes with spatial analysis to monitor oxygen consumption and fungal growth patterns.
- Assessed fungal metabolic activity and movement in the presence and absence of tomato roots.
Main Results:
- Fungal metabolism, indicated by oxygen consumption, significantly increased within hours of inoculation.
- Statistical analysis demonstrated directed growth of *Fol* towards tomato roots.
- In the absence of roots, fungal elements exhibited random Brownian motion.
Conclusions:
- Tomato roots actively influence the metabolic activity and directional growth of *Fusarium oxysporum* f. sp. *lycopersici*.
- The combination of planar optodes and spatial analysis provides a powerful method for dissecting early root-pathogen dynamics.
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