Related Experiment Video
Updated: Feb 7, 2026

Rapid Isolation of Single Cells from Mouse and Human Teeth
Published on: October 28, 2021
The Rate of Demineralization in the Teeth Prepared by Bur and Er:YAG Laser
Mahdi Abbasi1, Afrooz Nakhostin2, Fatemeh Namdar3
1Assistant Professor, Department of Restorative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Abstract:
Introduction: The present in vitro study evaluated the recurrence rate of caries following cavity preparations with bur (conventional technique) and irradiation by Erbium:Yttrium- Aluminum-Garnet (Er:YAG) laser through micro hardness test. Methods: A total of 72 human extracted molars were randomly divided into 3 groups and class 5 cavities were prepared on them with 3 different methods: G1) conventional bur, G2) Er:YAG laser irradiation alone and G3) laser irradiation + laser treatment. The specimens were immersed in the artificial caries solution with pH of 2.0 and 5.0 (12 days) and then immersed in re-mineralizing solution with pH of 7.0 (25 days). The specimens were longitudinally sectioned and their Vickers micro hardness was determined. Data were statistically analyzed by means of three-way analysis of variance (ANOVA) and Tukey multiple comparisons tests. Results: The micro hardness of the samples was affected by substrate type (enamel and dentin) and low values were achieved in dentin (P<0.001). Moreover, no significant difference was observed between preparation methods by bar and laser irradiation alone (P ≤0.499). Although laser irradiation + laser treatment decreased micro hardness of enamel compared to other methods. In dentin samples, different methods of preparation showed no significant effect on micro hardness (P ≤0.874). Conclusion: Due to the similar values of micro hardness following G1 and G2, it seems that Er:YAG laser alone is as much effective as the conventional bur to prevent recurrence caries. However, because of the high prices of laser instruments, bur preparations can be done commonly.
Related Concept Videos
Teeth
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin...
Reaction Rate
The mathematical representation of the change in the concentration of reactants and products, over time, is the rate...
Related Rates
Speciation Rates
Measuring Reaction Rates
Rate-Determining Steps
In a multistep reaction mechanism, one of the elementary steps progresses significantly slower than the others. This slowest step is called the rate-limiting step (or rate-determining step). A reaction cannot proceed faster than its slowest step, and hence, the rate-determining step limits the overall reaction rate.
The concept of rate-determining step can be understood from the analogy of a 4-lane freeway with a short-stretch of traffic-bottleneck caused due to...

