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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
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CRISPR-Cas9 therapies in experimental mouse models of cancer

Diogo Estêvão1,2, Natália Rios Costa1, Rui Gil da Costa3,4

  • 1Molecular Oncology & Viral Pathology Group, IPO Porto Research Center (CI-IPOP), Portuguese Oncology Institute of Porto (IPO Porto), 4200-072 Porto, Portugal Porto, Portugal.

Future Oncology (London, England)
|July 21, 2018
PubMed

Insights

CRISPR-Cas9 gene editing offers vast therapeutic potential for cancer, with recent advances in animal models and proof-of-concept studies. Challenges remain in translating this powerful research tool into clinical applications.

Area of Science:

  • Biotechnology
  • Genetics
  • Molecular Biology

Background:

  • CRISPR-Cas9 is a bacterial defense mechanism repurposed as a precise genome-editing tool.
  • Its applications include correcting mutations, inactivating oncogenes, and activating tumor suppressor genes.

Purpose of the Study:

  • To review recent advances in CRISPR-Cas9 applications for animal cancer models.
  • To discuss the development of CRISPR-Cas9-based therapies and proof-of-concept studies.
  • To identify challenges facing CRISPR-Cas9 as a therapeutic tool.

Main Methods:

  • Review of recent scientific literature on CRISPR-Cas9 in cancer research.
  • Analysis of proof-of-concept studies for CRISPR-Cas9-based therapies.
  • Discussion of challenges and future potential.

Main Results:

  • CRISPR-Cas9 is a highly precise and efficient genome-editing technology.
  • Significant progress has been made in utilizing CRISPR-Cas9 in animal cancer models.
  • Proof-of-concept studies show promise for CRISPR-Cas9-based cancer therapies.

Conclusions:

  • CRISPR-Cas9 demonstrates substantial potential for cancer therapy development.
  • Further research is needed to overcome challenges and realize its full therapeutic application.
  • The key question is whether CRISPR-Cas9 will transition from a research tool to a clinical reality.
Keywords:
CRISPR-Cas9cancer modelinggenome-editingmouse modelcancer

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