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Updated: Feb 7, 2026
05:33
Isolation of Soil Microorganisms Using iChip Technology
Published on: January 10, 2025
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Hymenobacter rufus sp. nov., a bacterium isolated from soil
Jeong-Eun Ohn1, Leonid N Ten2, Byung-Oh Kim1
11School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
Summary
A novel bacterial species, Hymenobacter rufus, was identified from a Korean soil sample. This Gram-negative bacterium exhibits unique red-pink colonies and distinct genetic and physiological characteristics, differentiating it from known Hymenobacter species.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Genomics
Background:
- The genus Hymenobacter comprises Gram-negative bacteria found in diverse environments.
- Accurate taxonomic classification is crucial for understanding microbial diversity and function.
- Soil ecosystems harbor a vast array of uncharacterized microorganisms.
Purpose of the Study:
- To isolate and characterize a novel bacterial strain from a soil sample.
- To determine the phylogenetic position of the isolate within the genus Hymenobacter.
- To propose a new species designation based on polyphasic taxonomic data.
Main Methods:
- Isolation and cultivation of bacteria from soil samples.
- 16S rRNA gene sequence analysis for phylogenetic identification.
- Physiological and biochemical characterization (Gram staining, colony color, growth conditions).
- Chemotaxonomic analyses (respiratory quinone, fatty acids, polar lipids).
- Genomic DNA G+C content determination.
- DNA-DNA hybridization for genetic relatedness assessment.
Main Results:
- A novel Gram-negative, short-rod-shaped bacterium (S1-2-2-6T) with red-pink colonies was isolated.
- 16S rRNA gene sequence analysis placed the strain within the genus Hymenobacter, with highest similarity to H. terrae DG7AT (98.2%).
- Physiological tests revealed growth between 4-30°C, pH 6-8, and 0-0.5% NaCl.
- Chemotaxonomic data included menaquinone-7 as the predominant respiratory quinone and phosphatidylethanolamine as the major polar lipid.
- DNA-DNA hybridization values with related Hymenobacter species were below 40%, supporting distinct species status.
- The genomic DNA G+C content was 60.7 mol%.
Conclusions:
- Strain S1-2-2-6T represents a novel species within the genus Hymenobacter.
- The proposed name for this new species is Hymenobacter rufus sp. nov.
- The type strain is S1-2-2-6T (=KCTC 52736T=JCM 32196T).
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