Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

2.4K
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
2.4K
Types Of Column Chromatography01:29

Types Of Column Chromatography

13.8K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
13.8K
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

9.0K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
9.0K
Diffusion on Chromatography Columns01:07

Diffusion on Chromatography Columns

1.3K
In column chromatography, when an analyte is introduced as a narrow band at the top of the column, the solutes begin to separate and broaden, developing a Gaussian profile. This broadening occurs due to various factors, such as longitudinal diffusion.
Longitudinal diffusion occurs when the solute molecules in the mobile phase diffuse from the more concentrated center of the chromatographic band to the more dilute regions on either side, both towards and against the flow direction. This...
1.3K
Gas Chromatography: Introduction01:13

Gas Chromatography: Introduction

4.0K
Gas chromatography (GC) is a technique for separating and analyzing volatile compounds in a sample. Its primary purpose is to identify and quantify components in complex mixtures, making it essential in fields such as environmental analysis, pharmaceuticals, and petrochemicals. GC is also called vapor-phase chromatography (VPC) or gas-liquid partition chromatography (GLPC).
In GC,  a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a...
4.0K
Gas Chromatography: Overview of Detectors01:13

Gas Chromatography: Overview of Detectors

2.0K
Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flavonoids as Dual Topoisomerase I and II Inhibitors: Mechanistic Insights and Emerging Anticancer Strategies.

Chemistry & biodiversity·2026
Same author

Silicon Dioxide Multi-Mode Interference Spectrometers.

Micromachines·2026
Same author

Effects of plate interface frictional heterogeneities on earthquake cycle dynamics in subduction zones.

Scientific reports·2026
Same author

Review of Recent Optofluidic Devices.

Micromachines·2026
Same author

Analog Control of Reconfigurable GHz Resonances from Chiral Spin Texture Ensembles.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Generating actionable insights to support point-of-care suicide risk decision-making in a safety-net healthcare system: a machine learning approach to predicting dynamic risk of intentional self-harm.

BMJ open·2026

Related Experiment Video

Updated: Feb 7, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.9K

Microchip gas chromatography columns, interfacing and performance.

Abhijit Ghosh1, Carlos R Vilorio2, Aaron R Hawkins2

  • 1Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.

Talanta
|July 22, 2018
PubMed
Summary
This summary is machine-generated.

Microchip gas chromatography offers potential but faces challenges in column design and interfacing. Overcoming these hurdles is key to wider adoption and application of this technology.

Keywords:
Column performanceGas chromatographyInterfacing technologyMicrochipMicrofabrication

More Related Videos

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography
08:22

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography

Published on: May 15, 2020

8.1K
Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

45.0K

Related Experiment Videos

Last Updated: Feb 7, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.9K
Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography
08:22

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography

Published on: May 15, 2020

8.1K
Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

45.0K

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Chemical Engineering

Background:

  • Planar (microchip) gas chromatography has been investigated for nearly 40 years.
  • Significant advancements have been made in producing and using these columns.

Purpose of the Study:

  • To review the historical development of microchip gas chromatography.
  • To identify key challenges hindering commercialization and broader application.
  • To stimulate future research and development for technological advancement.

Main Methods:

  • Literature review of investigations into planar gas chromatographic columns.
  • Analysis of practical constraints affecting performance and applications.
  • Identification of areas requiring innovative solutions.

Main Results:

  • Despite extensive research, practical limitations persist.
  • Non-ideal column geometries, dead volume, and interfacing issues impede progress.
  • Widespread commercialization remains limited due to these challenges.

Conclusions:

  • Continued research is needed to address existing limitations in microchip gas chromatography.
  • Creative approaches are essential to accelerate development.
  • Overcoming technical barriers will unlock the full potential of this technology.