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Published on: January 26, 2019
Plasmolipin, PmPLP1, from Penaeus monodon is a potential receptor for yellow head virus infection
Watchalaya Matjank1, Sirikwan Ponprateep2, Vichien Rimphanitchayakit3
1Applied Analytical Chemistry Research Unit, Department of Chemistry, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Chalongkrung Road, Bangkok, 10520, Thailand.
Abstract:
Plasmolipin has been characterized as a cell entry receptor for mouse endogenous retrovirus. In black tiger shrimp, two isoforms of plasmolipin genes, PmPLP1 and PmPLP2, have been identified from the Penaeus monodon EST database. The PmPLP1 is highly up-regulated in yellow head virus (YHV)-infected shrimp. Herein, the function of PmPLP1 is shown to be involved in YHV infection. The immunoblotting and immunolocalization showed that the PmPLP1 protein was highly expressed and located at the plasma membrane of gills from YHV-infected shrimp. Moreover, the PmPLP1 expressed in the Sf9 insect cells resided at the cell membrane rendering the cells more susceptible to YHV infection. Using the ELISA binding and mortality assays, the synthetic external loop of PmPLP1 was shown to bind the purified YHV and neutralize the virus resulting in the decrease in YHV infection. Our results suggested that the PmPLP1 was likely a receptor of YHV in shrimp.
Insights
Plasmolipins act as cell entry receptors. In shrimp, PmPLP1 is crucial for yellow head virus (YHV) infection, binding and neutralizing the virus, suggesting it
Area of Science:
- Aquaculture
- Virology
- Molecular Biology
Background:
- Plasmolipin is known as a cell entry receptor for mouse endogenous retrovirus.
- Two plasmolipin gene isoforms, PmPLP1 and PmPLP2, were identified in black tiger shrimp (Penaeus monodon).
- PmPLP1 expression is significantly increased in shrimp infected with yellow head virus (YHV).
Purpose of the Study:
- To investigate the role of PmPLP1 in YHV infection in shrimp.
- To determine the localization and function of the PmPLP1 protein.
- To assess the potential of PmPLP1 as a YHV receptor.
Main Methods:
- Gene identification from Penaeus monodon EST database.
- Immunoblotting and immunolocalization to detect PmPLP1 protein expression and location.
- Expression of PmPLP1 in Sf9 insect cells to assess YHV susceptibility.
- ELISA binding assays and mortality assays to evaluate virus-host interaction.
Main Results:
- PmPLP1 protein is highly expressed and localized to the plasma membrane of gills in YHV-infected shrimp.
- PmPLP1 expression in insect cells increases susceptibility to YHV infection.
- A synthetic external loop of PmPLP1 binds purified YHV.
- Binding of the synthetic loop neutralizes YHV, reducing infection.
Conclusions:
- PmPLP1 plays a significant role in YHV infection.
- PmPLP1 is likely a YHV receptor in shrimp.
- Targeting PmPLP1 may offer a strategy for YHV control in aquaculture.
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