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Updated: Feb 7, 2026

Cannula Implantation into the Cisterna Magna of Rodents
Published on: May 23, 2018
Standardized Method for Intra-Cisterna Magna Delivery Under Fluoroscopic Guidance in Nonhuman Primates
Nathan Katz1, Tamara Goode1, Christian Hinderer1
1Gene Therapy Program, Department of Medicine, University of Pennsylvania , Perelman School of Medicine, Philadelphia, Pennsylvania.
Abstract:
Intrathecal delivery of adeno-associated virus vectors and other therapeutics are currently being evaluated for the treatment of central nervous system sequelae of lysosomal storage diseases, motor neuron diseases, and neurodegenerative diseases. As products transition from preclinical to clinical studies, a standardized and clinically relevant method of intrathecal delivery is increasingly germane. Here, we describe a method of intrathecal delivery via suboccipital puncture into the cisterna magna under fluoroscopic guidance in nonhuman primates. This procedure is suitable for use in good laboratory practice compliant studies, has an excellent safety profile, and is highly similar to the procedure currently being explored for use in humans.
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