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Cultivation and characterization of three strains of murine rotavirus
Abstract:
Three distinct strains of murine rotavirus were adapted to growth in cell culture. These strains are genetically related but not identical; they are serotypically heterogeneous. The cultivatable strains were substantially more infectious (approximately 10(6)-fold) for suckling mice than heterologous simian rotaviruses were. Homologous murine rotavirus strains spread from inoculated to uninoculated litter mates and caused diarrhea, while heterologous rotaviruses did not spread and cause illness.
Insights
Cultivatable murine rotavirus strains showed significantly higher infectivity and transmissibility in suckling mice compared to simian rotaviruses. These adapted strains effectively spread between littermates, causing diarrhea.
Area of Science:
- Virology
- Infectious Diseases
- Animal Models
Background:
- Murine rotaviruses are important pathogens in animal models.
- Adaptation of rotaviruses to cell culture is crucial for research.
- Understanding strain-specific infectivity is key to disease control.
Purpose of the Study:
- To adapt distinct murine rotavirus strains for cell culture growth.
- To compare the infectivity and pathogenicity of adapted murine rotavirus strains with heterologous simian rotaviruses in suckling mice.
Main Methods:
- Adaptation of three distinct murine rotavirus strains to cell culture.
- Inoculation of suckling mice with adapted murine rotavirus strains and heterologous simian rotaviruses.
- Assessment of viral spread and illness (diarrhea) in inoculated and uninoculated littermates.
Main Results:
- Successfully adapted three genetically related but serotypically heterogeneous murine rotavirus strains to cell culture.
- Adapted murine rotavirus strains demonstrated approximately 10^6-fold higher infectivity in suckling mice compared to simian rotaviruses.
- Homologous murine rotavirus strains exhibited efficient spread to littermates and caused diarrhea, unlike heterologous strains.
Conclusions:
- Cell culture-adapted murine rotavirus strains are highly infectious and transmissible in a homologous animal model.
- These findings highlight the importance of strain characteristics in rotavirus pathogenesis and host adaptation.
- The adapted strains serve as valuable tools for studying rotavirus biology and developing countermeasures.