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[Determination of Histamine by a Rapid Fluorescamine-UPLC Method]
Yuuki Murakami1, Natsuki Nakai1, Naoko Ando1
1Nara Prefecture Institute of Health.
A new method rapidly determines histamine in food using ultra-performance liquid chromatography and fluorescamine derivatization. This technique aids in quickly assessing food poisoning risks from contaminated seafood and other products.
Area of Science:
- Analytical Chemistry
- Food Science
- Biochemistry
Background:
- Histamine accumulation in food can cause foodborne illness.
- Accurate and rapid detection of histamine is crucial for public health.
- Existing methods for histamine determination can be time-consuming.
Purpose of the Study:
- To develop a rapid and efficient method for histamine determination in various food matrices.
- To validate the method's accuracy and reliability for food safety applications.
- To provide a tool for quick assessment of food poisoning outbreaks.
Main Methods:
- Histamine extraction from homogenized food samples using trichloroacetic acid.
- Direct derivatization of histamine with fluorescamine without solid-phase extraction.
- Analysis using ultra-performance liquid chromatography (UPLC) with a phosphate-acetonitrile mobile phase.
Main Results:
- The developed UPLC method provides rapid histamine determination.
- High recovery rates (95.8-117.7%) were achieved for histamine in fish, seasoning, and fish products.
- The method avoids complex procedures like solid-phase extraction.
Conclusions:
- The developed method is suitable for the rapid analysis of histamine in food.
- This technique can be valuable for timely evaluation of potential food poisoning outbreaks.
- The method offers a reliable alternative for routine histamine monitoring in food safety.
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