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Updated: Feb 7, 2026

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
Complete genome sequence of Enterococcus durans KLDS6.0933, a potential probiotic strain with high cholesterol
Bailiang Li1, Smith Etareri Evivie1,2, Da Jin1
11Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, 150030 People's Republic of China.
Background:
Enterococci are commensal bacteria in the mammalian gastrointestinal tract which play an important role in the production of various fermented foods. Thus, certain enterococcal strains are commonly used as probiotics to confer health benefits to human and animals. Enterococcus durans KLDS6.0933 is a potential probiotic strain with high cholesterol removal ability, which was isolated from traditional naturally fermented cream in Inner Mongolia of China. To better understand the genetic basis of the probiotic properties of this strain, the whole-genome sequence was performed using the PacBio RSII platform.
Results:
Enterococcus durans KLDS6.0933 contains a circular chromosome of 2,867,028 bp, two plasmids of 163,286 bp and 41,490 bp, respectively. Within the 2704 predicted genes, genes involved with acid, bile and oxidative stress resistance were identified. Bile salt hydrolase (BSH, LIANG_RS13510), a cholesterol removal enzyme identified in the E. durans KLDS6.0933 genome is different from that of other Enterococcus strains. Furthermore, unlike other Enterococcus strains, E. durans KLDS 6.0933 can facilitate the complete biosynthesis pathway of l-tryptophan.
Conclusions:
In silico analysis confirmed the probiotic properties of E. durans KLDS6.0933 and may help us exploit the potential applications of E. durans KLDS6.0933 as an industrially important strain.
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