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Peptide inhibitors of Macrobrachium rosenbergii nodavirus

Qiu Xian Thong1, Chuan Loo Wong1, Man Kwan Ooi1,2

  • 11​Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

The Journal of General Virology
|July 26, 2018
PubMed

Insights

Researchers identified two novel peptides, HTKQIPRHIYSA and VSRHQSWHPHDL, that inhibit Macrobrachium rosenbergii nodavirus (MrNv) entry into cells. These peptides show potential as antiviral agents against white tail disease (WTD) in prawns.

Area of Science:

  • Aquaculture
  • Virology
  • Biotechnology

Background:

  • Macrobrachium rosenbergii nodavirus (MrNv) causes significant economic losses in giant freshwater prawn aquaculture due to white tail disease (WTD).
  • Currently, no effective antiviral treatments exist for MrNv, necessitating the discovery of novel therapeutic agents.

Purpose of the Study:

  • To identify and characterize novel molecules with anti-MrNv activity.
  • To evaluate the potential of identified peptides as therapeutic agents against WTD.

Main Methods:

  • Biopanning of a 12-mer phage-displayed peptide library against MrNv virus-like particles (VLPs).
  • Equilibrium binding assays to determine dissociation constants (KDrel) of selected peptides.
  • In-cell ELISA and MTT assays to assess peptide efficacy in inhibiting MrNv VLP entry and improving cell viability.
  • Real-time RT-PCR to quantify MrNv load in infected cells.

Main Results:

  • Two dominant peptides, HTKQIPRHIYSA (KDrel = 92.4±22.8 nM) and VSRHQSWHPHDL (KDrel = 12.7±3.8 nM), were identified.
  • Both peptides inhibited MrNv VLP entry into Sf9 cells with IC50 values of 30.4±3.6 µM and 26.5±8.8 µM, respectively.
  • A combination of both peptides showed enhanced inhibition (IC50 = 4.9±0.4 µM), increased cell viability to 97%, and significantly reduced MrNv load.

Conclusions:

  • The identified peptides, HTKQIPRHIYSA and VSRHQSWHPHDL, demonstrate potent antiviral activity against MrNv.
  • These peptides represent promising lead compounds for the development of novel antiviral therapies for white tail disease in aquaculture.
Keywords:
affinity selectionpeptide inhibitorsphage displayvirus-host interactionwhite tail disease

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