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Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles
Published on: March 15, 2012
Expression and characterization of a pectin methylesterase from Aspergillus niger ZJ5 and its application in fruit
Zhiwei Zhang1, Junshuai Dong1, Deqing Zhang2
1College of Forestry, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Abstract:
A pectin methylesterase gene, pme-zj5a, from Aspergillus niger ZJ5 was cloned and high-level expressed in Pichia pastoris. The highest PME activity was 71.11 U/ml after induction with methanol for 20 h at 30 °C. The molecular mass of purified PME-ZJ5A was estimated to be 37 kDa by SDS-PAGE, and its Km, Vmax and kcat values of PME-ZJ5A were determined to be 3.27 mg/ml, 5.36 μmol/min/mg, and 22.33 s-1 with pectin. Purified recombinant PME-ZJ5A exhibited optimal activity at pH 3.8 and 45 °C. It retained more than 60% of its maximum activity at 10 °C. Moreover, recombinant PME-ZJ5A can increase the transmittance of pineapple juice by 60.8%, and increase the firmness of pineapple cubes nearly double when combined with CaCl2, which showed good potential in fruit processing.
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