A pH-metrical method of determining glucose-6-phosphatase activity

Area of Science:

• Biochemistry
• Enzymology

Background:

• Glucose-6-phosphatase is a key enzyme in glucose metabolism.
• Accurate measurement of its activity is crucial for understanding metabolic disorders.

Purpose of the Study:

• To develop a simple, rapid, and reproducible method for determining glucose-6-phosphatase activity.
• To establish a reliable assay for routine laboratory use.

Main Methods:

• Enzyme activity determination based on pH changes.
• Monitoring proton disappearance during glucose 6-phosphate hydrolysis using a pH meter and recorder.
• Utilizing the pK shift of phosphate species from 6.1 to 6.9.

Main Results:

• The method accurately reflects glucose-6-phosphatase activity through measurable pH shifts.
• The assay is simple, rapid, and reproducible.
• The observed alkalization is directly linked to enzyme activity.

Conclusions:

• A novel, efficient method for quantifying glucose-6-phosphatase activity has been established.
• This assay is suitable for routine diagnostic and research applications.
• The pH-based detection offers a practical approach to enzyme activity measurement.