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Application of CRISPR-Cas9 for Long Noncoding RNA Genes in Cancer Research

Shuai Zhen1,2, Xu Li1,2

  • 11 Center for Translational Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Human Gene Therapy
|July 27, 2018
PubMed

Insights

Long noncoding RNAs (LncRNAs) are key in cancer. The CRISPR-Cas9 gene-editing tool offers new ways to study and modify LncRNA functions, advancing cancer research and potential therapies.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Long noncoding RNAs (LncRNAs) are crucial regulators in cancer development.
  • The CRISPR-Cas9 system is a powerful genome editing technology.

Purpose of the Study:

  • To review the functions and mechanisms of LncRNAs targeted by CRISPR-Cas9.
  • To discuss CRISPR-Cas9 as a strategy for LncRNA expression modification.

Main Methods:

  • Literature review of studies utilizing CRISPR-Cas9 for LncRNA research.
  • Analysis of LncRNA targeting and functional evaluation methods.

Main Results:

  • CRISPR-Cas9 effectively targets LncRNAs for functional studies.
  • CRISPR-Cas9 enables precise modification of LncRNA expression.

Conclusions:

  • CRISPR-Cas9 is a versatile tool for investigating LncRNA roles in cancer.
  • CRISPR-Cas9 delivery strategies are advancing for clinical applications.
Keywords:
CRISPR-Cas9LncRNAcancer

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