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A single high-fat meal alters human soluble RAGE profiles and PBMC RAGE expression with no effect of prior aerobic

Kelly N Z Fuller1, Rudy J Valentine2, Edwin R Miranda1,3

  • 1Department of Kinesiology and Nutrition, University of Illinois at Chicago, Chicago, Illinois.

Physiological Reports
|July 27, 2018
PubMed

Insights

A high-fat meal reduced soluble receptor for advanced glycation endproducts (sRAGE) and increased its expression on cells, but prior exercise had no effect on these metabolic changes.

Area of Science:

  • Metabolic Health
  • Inflammation Research
  • Exercise Physiology

Background:

  • High-fat diets (HFDs) trigger inflammation and metabolic diseases like diabetes and atherosclerosis.
  • The receptor for advanced glycation endproducts (RAGE) is crucial in metabolic disease; its soluble form (sRAGE) offers protection.
  • The role of RAGE in the postprandial state and the influence of exercise remain unclear.

Purpose of the Study:

  • To investigate the impact of a single high-fat meal (HFM) on peripheral blood mononuclear cell (PBMC) RAGE biology.
  • To determine if acute aerobic exercise modifies the postprandial response to an HFM concerning RAGE.
  • To analyze changes in soluble RAGE isoforms (sRAGE, esRAGE, cRAGE) and PBMC RAGE, ADAM10, TLR4, and MyD88 expression.

Main Methods:

  • Twelve healthy males consumed an HFM on two separate occasions.
  • One HFM was preceded by acute aerobic exercise (16-18 hours prior); the other was not.
  • Blood samples were analyzed for sRAGE, esRAGE, and PBMC expression of RAGE, ADAM10, TLR4, and MyD88.

Main Results:

  • The HFM significantly decreased circulating sRAGE, esRAGE, and calculated cleaved RAGE (cRAGE) levels.
  • Conversely, the HFM significantly increased RAGE protein expression on PBMCs.
  • No significant effects of the HFM or prior exercise were observed on PBMC TLR4, MyD88, or ADAM10 expression or activity.

Conclusions:

  • PBMC RAGE and soluble RAGE isoforms are involved in the postprandial response to HFM in healthy males.
  • Acute aerobic exercise did not alter the observed changes in PBMC RAGE biology or circulating sRAGE after an HFM.
  • Further research is needed to elucidate the mechanisms behind HFM-induced RAGE changes and reduced soluble RAGE.
Keywords:
ADAM10MyD88Toll-like receptor 4postprandial

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