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Lnc-SNHG1 Activates the TGFBR2/SMAD3 and RAB11A/Wnt/β-Catenin Pathway by Sponging MiR-302/372/373/520 in Invasive

Heyuan Wang1,2, Guixia Wang1, Yufei Gao3

  • 1Department of Endocrinology and Metabolism, The First Hospital of Jilin University, Changchun, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|July 27, 2018
PubMed
Abstract

Insights

Long noncoding RNA SNHG1 promotes pituitary tumor growth by inhibiting miR-302/372/373/520. This lncRNA acts as a therapeutic target for invasive pituitary tumors.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are key regulators in diseases like cancer.
  • lncRNAs can act as competing endogenous RNAs (ceRNAs) to modulate microRNA (miRNA) activity.
  • Pituitary tumors are complex neoplasms where regulatory mechanisms are still being elucidated.

Purpose of the Study:

  • To investigate the role of lnc-SNHG1 in pituitary tumor progression.
  • To explore the molecular mechanisms underlying lnc-SNHG1's function in pituitary tumors.
  • To assess lnc-SNHG1 as a potential therapeutic target.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • Cellular assays (MTT, cell count, transwell, flow cytometry) for proliferation, migration, invasion, and apoptosis.
  • Molecular analyses including Western blot, immunofluorescence, and luciferase reporter assays.
  • In vivo tumor xenograft models to assess tumor growth.

Main Results:

  • lnc-SNHG1 is overexpressed in invasive pituitary tumors and promotes proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT).
  • lnc-SNHG1 overexpression inhibits miR-302/372/373/520 and upregulates target genes TGFBR2 and RAB11A.
  • lnc-SNHG1 activates TGFBR2/SMAD3 and RAB11A/Wnt/β-catenin pathways via miRNA sponging.

Conclusions:

  • lnc-SNHG1 significantly promotes pituitary tumor progression.
  • lnc-SNHG1 acts as a ceRNA by sponging miR-302/372/373/520, thereby activating oncogenic pathways.
  • lnc-SNHG1 represents a promising therapeutic target for invasive pituitary tumors.
Keywords:
Lnc-SNHG1Pituitary tumorRAB11ASMAD3TGFBR2Wnt/β-catenin

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