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Updated: Feb 7, 2026

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
Published on: November 24, 2017
Water solubility is essential for fluorescent probes to image hypochlorous acid in live cells
Panfei Xing1, Zhe Zhang, Yiming Niu
1State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau SAR. CMWang@umac.mo.
Abstract:
We synthesised four probes and compared their HClO-detecting ability in different solvents. The data showed that only hydrophilic probes could sensitively and accurately detect HClO in live cells. Meanwhile, the addition of organic solvents, as is commonly practised, weakens the oxidising capacity of HClO and thus generates inaccurate outcomes.
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